The expression and cellular distribution of Id2 in myoblast C2C12 under different stimuli
LAI Gui-hua, HU Xiao-fang, ZHANG Xiang, LU Xing-hao, YU Peng, YU Lei
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (2) : 172-176.
The expression and cellular distribution of Id2 in myoblast C2C12 under different stimuli
Objective TO observe the expression and distribution of Id2 after myoblasts were induced to proliferation, apoptosis and differentiation with various stimuli. Methods Cultured C2C12 cells were treated with 25~300 µmol/L different concentration of H2O2, 100 ng/ml or 500 ng/ml of LPS, and 2% horse serum to induce proliferation, apoptosis or differentiation. After treatment, RT-PCR and immunofluorescence were used to detect the expression level and the cellular distribution of Id2 in C2C12 cells. Results After treatment with 25 µmol/l or 50 µmol/l of H2O2, expression of Id2 in C2C12 cells was 80.5% and 55.5% higher than control cells, and Id2 were present evenly in the both nucleus and cytoplasm. LPS of 100 ng/ml and 500 ng/ml also induced Id2 expression, 21.7% and 40.2% higher than control cells，but at a weaker level, and the majority of Id2 was in the nucleus of C2C12, with the detectable signals in the cytoplasm. On the contrary, with differentiation of C2C12 cells after treatment with 2% horse serum, Id2 expression decreased and most Id2 migrated into cytoplasm. Conclusion Different expression and distribution of Id2 could be observed after induction of myoblasts to proliferation, apoptosis and differentiation with various stimuli. And the result has demonstrated that Id2 could regulate regeneration of skeletal muscle. Ithas indicated that Id2 is a very impossible regulatory factor of regeneration of skeletal muscle after injury.
Myoblast / Id2 / Stimulation / Expression / Distribution
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