Expression changes of pEGFR after decompression of compressed spinal cord injury in rats
GONG Rui, SUN Shan-quan, ZHONG Yuan, ZHANG Wei, ZHAO Qi, MU Ke-jie, XUE Jun
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (2) : 166-171.
Expression changes of pEGFR after decompression of compressed spinal cord injury in rats
Objective To investigate the changes of pEGFR, pAkt1 expression in rat after decompression of compressed spinal cord injury(CSCI), and its correlation with nerve structure and function change，providing experimental basis for the development of therapeutic strategies and the development of medication for the treatment of CSCI after decompression. Methods The CSCI model was established first with a self-made device, which then underwent spinal decompression. The motor functions were monitored by Basso, Beattie & Bresnahanlocomotor rating scale. The pathological changes in axonal myelinated fibers were estimated by luxol fast blue (LFB). Epidermal growth factor receptor (EGFR), and phosphorylated Akt1 (pAkt1) were detected by double-labeling immunofluorescence and western blotting assays. Results The motor functions and number of myelinated nerve fibers were increased along with time extending after decompression. The expression of EGFR and pAkt1 was also increased after decompression, which was consistent with the changes of motor functions and number of myelinated nerve fibers. Conclusion The structure and function of the injured nerve can be restored to a certain extent, and the expression of pEGFR is closely related to this phenomenon after decompression. It suggests that the activation of EGFR is involved in the endogenous repair of CSCI after decompression.
Compressed spinal cord injury / Neural myelin sheaths / Demyelination / EGFR / pAkt1
[1] Huang SQ, Tang CL, Sun SQ, et al. Demyelination initiated by oligodendrocyte apoptosis through enhancing endoplasmic reticulum-mitochondria interactions and Id2 expression after compressed spinal cord injury in rats [J].CNS Neurosci Ther, 2014,20 (1):20-31.
[2] 伍修宇, 孙善全, 钟小燕, 张波等. 脊髓压迫性损伤神经纤维溃变和髓鞘碱性蛋白的表达变化 [J].中国临床解剖学杂志, 2012, 30(6): 658-662.
[3] All AH, Bazley FA, Gupta S, et al. Human embryonic stem cell-derived oligodendrocyte progenitors aid in functional recovery of sensory pathways following contusive spinal cord injury [J].PLoS One, 2012,7 (10):e47645.
[4] Gamett DC, Tracy SE, Robinson HL. Differences in sequences encoding the carboxyl-terminal domain of the epidermal growth factor receptor correlate with differences in the disease potential of viral erbB genes [J]. Proc Natl Acad Sci U S A, 1986,83 (16):6053-6057.
[5] Hayakawa-Yano Y, Nishida K, Fukami S, et al. Epidermal growth factor signaling mediated by grb2 associated binder1 is required for the spatiotemporally regulated proliferation of olig2-expressing progenitors in the embryonic spinal cord [J].Stem Cells, 2007,25(6):1410-1422.
[6] Flores AI, Mallon BS, Matsui T, et al. Akt-mediated survival of oligodendrocytes induced by neuregulins [J]. J Neurosci, 2000, 20 (20):7622-7630.
[7] 梁益建,孙善全,汪克建,等. 大鼠脊髓慢性压迫性损伤实验模型的建立 [J].中国临床解剖学杂志，2006，24(3): 318-324.
[8] Basso DM, Beattie MS, Bresnahan JC. A sensitive and reliable locomotor rating scale for open field testing in rats [J].J Neurotrauma,1995, 12 (1):1-21.
[9] Ouyang H, Sun W, Fu Y, et al. Compression induces acute demyelination and potassium channel exposure in spinal cord [J].J Neurotrauma,2010, 27 (6):1109-1120.
[10] Takenouchi T, Setoguchi T, Yone K, et al. Expression of apoptosis signal-regulating kinase 1 in mouse spinal cord under chronic mechanical compression: possible involvement of the stress-activated mitogen-activated protein kinase pathways in spinal cord cell apoptosis [J].Spine,2008, 33 (18):1943-1950.
[11] Li N, Leung GK. Oligodendrocyte precursor cells in spinal cord injury: a review and update [J].Bio med Res Int, 2015, 2015:235195.
[12]Aguirre A, Dupree JL, Mangin JM, et al. A functional role for EGFR signaling in myelination and remyelination [J].Nat Neurosci,2007,10 (8):990-1002.
/
| 〈 |
|
〉 |
