Effect of nerve growth factor on survival of cross-boundary perforator flap in rats
YANG Xi，Li Guo-dong，WANG Teng，HE Xiao-qing，XU Yong-qing
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (2) : 160-165.
Effect of nerve growth factor on survival of cross-boundary perforator flap in rats
Objective To evaluate the nerve growth factor (NGF) effect on survival of dorsal cross-boundary perforator flap of SD rats. Methods 60 adult SD rats were divided into the experimental group and the control group equally，in the dorsum of which a cross-boundary perforator flap with an area of about 3 cm×10 cm was harvested. The experimental group was injected subcutaneously with NGF solution (10 nmol·ml-1·kg-1); the control group was injected with 0.1 mol/L PBS solution (1ml/kg). 3 and 7 days after surgery, VEGF and CD34 of the local tissues of flap were tested via Blot Western；Angiography was performed by the micro-CT to detect Three-dimensional morphological alteration of microvasculature using factorial design analysis of vascular volume and total length. At day 7, survival area of the flap was measured before the observation of expression of KDR and TrkA in vasculature by immunohistochemical stain. Results Day 3, there was no statistically significant differences in the expression of VEGF between two groups (P=0.088); the expression of CD34 in the experimental group was higher than that in the control group (P=0.004); Day 7, there were higher expression of VEGF and CD34 in the experimental group (P<0.05)；Morphological analysis showed that there is an interaction between NGF and time in the increase of vascular volume (F=33.304, P<0.05) and the total length of vessels (F=8.493, P=0.01); Compared with the control group, the flap survival area of the experimental group was significantly higher (P<0.05). The expression of KDR in experimental group was markedly superior to the control group. Conclusion NGF can promote the survival of thecross-boundary perforator flap in SD rat.
Nerve growth factor / Cross-boundary flap / Perforator flap / Micro-computed tomography / Morphologic analysis
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