Effects of Schwann-like cells on the expression of NGF and BDNF in spinal ganglia of rats with sciatic nerve injury
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (1) : 48-51.
Effects of Schwann-like cells on the expression of NGF and BDNF in spinal ganglia of rats with sciatic nerve injury
Objective To investigate the effects of Schwann-like cells on the expression of NGF and BDNF in spinal ganglia of rats with sciatic nerve injury (SNI) and explore the protective effects of Schwann-like cells on spinal ganglion. Methods Firstly，the adipose derived stem cells (ADSCs) were differentiated into Schwann-like cells and the phenotype of the differentiated cells were identified, then the ADSCs and Schwann-like cells were implanted into the acellular nerve grafts (ANA), so the tissue engineering nerves were constructed. The rats were randomly divided into 3 groups: normal control group, ADSC group and Schwann-like cells group. The SNI model was established in rats of the latter two groups, and then the corresponding tissue engineering nerves were used to bridge the injured sciatic nerves. 4 Weeks after operation, Western Blot and Real-time PCR were used to detect the expression of NGF and BDNF protein and mRNA in spinal ganglia. Results ADSCs can be induced to differentiate into Schwann-like cells and expression of S100β and GFAP protein (markers of Schwann cells). In contrast with ADSC group, the expressions of NGF and BDNF protein and mRNA in Schwann-like cells group rats increased (P＜0.05). Conclusion Schwann-like cells can enhance the expression of NGF and BDNF in the spinal ganglia, so they can have protective effects on the spinal ganglia in rats with SNI.
Schwann-like cells / Spinal ganglia / Nerve growth factor / Brain-derived neurotrophic factor
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