Protective effect of chymase inhibitor on renal function in diabetic hamsters
ZHANG Meng-shu, LIU Jin-lei, LIU Xue-zheng，ZHOU Hong-li, ZHANG Rong-ming
Chinese Journal of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (6) : 661-667.
Protective effect of chymase inhibitor on renal function in diabetic hamsters
Objective To observe the protective effect of chymotrypsin inhibitors on diabetic hamster kidney, and to explore mechanism underlying the protection. Methods Diabetic hamsters was established by intraperitoneal injection of STZ. The animal chymotrypsin, RAS components: ACE, renin, ANG-I, ANG-II; oxidative stress levels: 8-OhdG, NOX-4, p22phox, and the expression of TGF-β1 were determined by molecular biologic techniques, immunohistochemistry, western blotting technique. Results Chymotrypsin inhibitors inhibited the increase of ANG-II in diabetic hamster kidney, but significantly reduced the level of 8-OHdG secretion. NOX-4 and p22phox staining indicated significantly increased products of oxidation products in diabetic glomerulus and tubules, which could be significantly inhibited by chymotrypsin inhibitors (P<0.01). Western blot confirmed that the level of NOX-4 and p22phox increased in the diabetic group than in the control group, and chymotrypsin inhibitor could significantly reduce its expression. Glomerular TGF-β1 expression in the diabetic group was higher than that in the control group, and the chymotrypsin inhibitor therapy could significantly reduce the levels of TGF-β1. Conclusions Chymotrypsin inhibitors can protect diabetic hamster kidney function, and the underlying mechanism is through reducing renal ANG-II levels and oxidative stress levels to protect diabetic kidney damage.
Chymase inhibitor / Oxidative stress / Kidney / Diabetic hamsters
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