The study of human adipose-derived stem cells in promoting the tendon-bone healing in a rabbit anterior cruciate ligament reconstruction model
CHEN Ping, QU Rong-mei, HAN Zhong-yu, YANG Yu-chao, YIN Yu-kun, TIAN Jing, ZHANG Li
Chinese Journal of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (6) : 643-646.
The study of human adipose-derived stem cells in promoting the tendon-bone healing in a rabbit anterior cruciate ligament reconstruction model
Objective To investigate whether human adipose-derived stem cells (hACSs) can promote tendon-bone healing. Methods Unilateral anterior cruciate ligament reconstruction using autologous semitendinosus tendons were conducted on 30 New Zealand rabbits(weighing 2~2.5kg), which were randomly divided into 2 groups. Rabbits in the experimental group were injected with hASCs in the tibial and femoral bone tunnels while rabbits in the control group were injected with equal normal saline. All experimental animals were sacrificed at 2 weeks, 4 weeks and 8 weeks after surgery. The specimens were harvested for gross observation and histological analysis. Results At 2 weeks after operation in the control group, a lot of inflammatory cells infiltrated the tendon-bone interface while the inflammatory cells decreased at 4 weeks. At 8 weeks postoperatively, sharpey’s fibers were found in the interface forming indirect insertion. In the experimental group, the tendon-bone interface was filled with fibro-cartilage cells and a few osteoblasts at 4 weeks after surgery. New bones can be found in the tendon-bone interface at 8 weeks postoperatively in the experimental group and formed the direct insertion. Conclusion hASCs can promote tendon-bone healing by forming a direct insertion in a rabbit anterior cruciate ligament reconstruction model.
Human adipose-derived stem cells / Anterior cruciate ligament reconstruction / Tendon-bone healing
[1] Lohmander LS, Englund PM, Dahl LL, et al. The long-term consequence of anterior cruciate ligament and meniscus injuries: osteoarthritis[J]. Am J Sports Med,2007,35(10):1756-1769.
[2] Spindler KP, Wright RW. Clinical practice. Anterior cruciate ligament tear[J]. N Engl J Med, 2008, 359(20):2135-2142.
[3] Griffin LY, Agel J, Albohm MJ, et al. Noncontact anterior cruciate ligament injuries: risk factors and prevention strategies[J]. J Am Acad Orthop Surg, 2000, 8(3):141-150.
[4] Anderson K, Seneviratne AM, Izawa K, et al. Augmentation of tendon healing in an intraarticular bone tunnel with use of a bone growth factor[J]. Am J Sports Med, 2001, 29(6):689-698.
[5] Sasaki K, Kuroda R, Ishida K, et al. Enhancement of tendon-bone osteointegration of anterior cruciate ligament graft using granulocyte colony-stimulating factor[J]. Am J Sports Med, 2008, 36(8):1519-1527.
[6] Kim JG, Kim HJ, Kim SE, et al. Enhancement of tendon-bone healing with the use of bone morphogenetic protein-2 inserted into the suture anchor hole in a rabbit patellar tendon model[J]. Cytotherapy, 2014, 16(6):857-867.
[7] Carragee EJ, Hurwitz EL, Weiner BK. A critical review of recombinant human bone morphogenetic protein-2 trials in spinal surgery: emerging safety concerns and lessons learned[J]. Spine J, 2011, 11(6):471-491.
[8] Lim JK, Hui J, Li L, et al. Enhancement of tendon graft osteointegration using mesenchymal stem cells in a rabbit model of anterior cruciate ligament reconstruction[J]. Arthroscopy, 2004, 20(9):899-910.
[9] Kanaya A, Deie M, Adachi N, et al. Intra-articular injection of mesenchymal stromal cells in partially torn anterior cruciate ligaments in a rat model[J]. Arthroscopy, 2007, 23(6):610-617.
[10] Toma JG, Akhavan M, Fernandes KJ, et al. Isolation of multipotent adult stem cells from the dermis of mammalian skin[J]. Nat Cell Biol, 2001, 3(9):778-784.
[11] Kern S, Eichler H, Stoeve J, et al. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue[J]. Stem Cells, 2006, 24(5):1294-1301.
[12] Rodeo SA, Suzuki K, Deng XH, et al. Use of recombinant human bone morphogenetic protein-2 to enhance tendon healing in a bone tunnel[J]. Am J Sports Med,1999, 27(4):476-488.
[13] Takigami J, Hashimoto Y, Yamasaki S, et al. Direct bone-to-bone integration between recombinant human bone morphogenetic protein-2-injected tendon graft and tunnel wall in an anterior cruciate ligament reconstruction model[J]. Int Orthop, 2015, 39(7):1441-1447.
[14] Zuk P A, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies[J]. Tissue Eng, 2001, 7(2):211-228.
[15] Hicok KC, Du Laney TV, Zhou YS, et al. Human adipose-derived adult stem cells produce osteoid in vivo[J]. Tissue Eng, 2004,10(3-4):371-380.
[16] Yoon E, Dhar S, Chun DE, et al. In vivo osteogenic potential of human adipose-derived stem cells/poly lactide-co-glycolic acid constructs for bone regeneration in a rat critical-sized calvarial defect model[J]. Tissue Eng, 2007, 13(3):619-627.
[17] Sheykhhasan M, Qomi RT, Ghiasi M. Fibrin scaffolds designing in order to human adipose-derived mesenchymal stem cells differentiation to chondrocytes in the presence of TGF-beta3[J]. Int J Stem Cells, 2015, 8(2):219-227.
[18] An C, Cheng Y, Yuan Q, et al. IGF-1 and BMP-2 induces differentiation of adipose-derived mesenchymal stem cells into chondrocytes-like cells[J]. Ann Biomed Eng, 2010, 38(4):1647-1654.
[19] Jeon O, Rhie JW, Kwon IK, et al. In vivo bone formation following transplantation of human adipose-derived stromal cells that are not differentiated osteogenically[J]. Tissue Eng Part A, 2008, 14(8):1285-1294.
[20] Karaoglu S, Celik C, Korkusuz P. The effects of bone marrow or periosteum on tendon-to-bone tunnel healing in a rabbit model[J]. Knee Surg Sports Traumatol Arthrosc, 2009,17(2):170-178.
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