BMSCs-BMP-2 composite with magnesium alloy rod in the repair of femoral head necrosis
ZHANG Yan-ru,ZHANG Hui, Kaka, ZHANG Ge-chen, SHANG Yan, SUN Hao, HUANG Wen-hua
Chinese Journal of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (5) : 528-533.
BMSCs-BMP-2 composite with magnesium alloy rod in the repair of femoral head necrosis
Objective To investigate the effect of BMSCs transfected BMP-2 composite with magnesium alloy rod in the repair of the femoral head necrosis. Methods Rabbit BMSCs was isolated, cultured, and successfully transfected pcDNA3.1-BMP-2 plasmid by electroporation. 40 rabbit femoral head necrosis was established by liquid nitrogen freezing method, and then were randomly divided into 4 groups of 10 animals each: the magnesium rod /BMSCs group, the magnesium rod group, the BMSCs group, and the blank group. The bio-magnesium alloy rod imported the BMSCs-BMP-2 plasmid pcDNA3.1. Repair effect was assessed post-operatively at 6 weeks and 12 weeks. Results Six weeks and 12 weeks after operation, the concentration of magnesium ion in blood and urine was tested by first, two, four, eight and twelve weeks, respectively. X-ray and HE staining at the sixth and twelfth week showed that the femoral head of magnesium rod /BMSCs group had the best recovery, and at the twelfth week, the magnesium alloy rod was completely absorbed; the femoral head necrosis in the magnesium rod group and BMSCs group was slower, and some of the femoral head necrosis was improved. Conclusion Implantation of composite magnesium alloy-BMSCs-BMP-2 gene has effect on delaying femoral head necrosis and improving repair.
BMSCs / BMP-2 / Magnesium alloy / Femoral head necrosis / Rabbits
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