Effect of dexamethasone on the expression of TREM-1 in acute lung injury
LUO Feng, ZHENG Hao-xiang, DONG Liang, LI Nian-wang, ZHOU Qin, JIA Zhe, HE Li-ping,SUN Guo-ying
Chinese Journal of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (5) : 523-527.
Effect of dexamethasone on the expression of TREM-1 in acute lung injury
Objective To investigate the effects of dexamethasone (Dex) on the TREM-1expression in acute lung injury(ALI) of mice induced by lipopolysaccharide (LPS). Methods Kunming mice (KM mice) were used as the research object and LPS (10 mg/kg) was used to induce acute lung injury model. After half an hour given different concentrations of Dex (5, 10, 20, 40 mg/kg) and selection of different time points (6, 12, 24 and 36 h) were observed. The lung tissue was observed by HE staining, RT-PCR was used to detect TREM-1 expression, and ELISA was used to detect the expression of sTREM-1 in bronchoalveolar lavage fluid of mice. Results Dex could time-dependently down-regulate the expression of lung tissue in the acute lung injury and in 6h treatment peak; Dexcould dose-dependently down-regulate TREM-1 expression in acute lung injury of lung tissue, and reached the peak at 10 mg /kg. Dex could decrease the protein level of sTREM-1 in bronchoalveolar lavage fluid of ALI mice. Conclusion Dex can down-regulate TREM-1 of lung tissues of ALI mice in a dose and time-dependent manner, and reduce myeloid cell membrane of TREM-1 in lung. Our research indicates that Dex may inhibit inflammatory cascade and protect lung tissue by regulation of TREM-1 expression.
Triggering receptor expressed on myeloid cells-1 / Acute lung injury / Dexamethasone;Lipopolysaccharide
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