Observe the expression of c-kit gene in intestinal cells of Cajal by establishing incomplete small intestinal obstruction models of guinea pig with silk method
YANG Zheng, LI Xin-rui, WANG Qiang, LIU Shuang, HUANG Qian, JI Zhen-hua
Chinese Journal of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (4) : 423-427.
Observe the expression of c-kit gene in intestinal cells of Cajal by establishing incomplete small intestinal obstruction models of guinea pig with silk method
Objective To investigatethe expression of c-kit gene in intestinal cells of Cajal by establishing incomplete small intestinal obstruction models in guinea pig with the silk method. Methods 43 adult healthy guinea pigs were selected and randomly divided into the normal group A, sham-operated group B, model groups（C, D, E）. Model groups were established for incomplete small intestinal obstruction by the means of silk method for 3 days. After taking out of the silk and closing the abdominal cavity, the abdominal cavity was opened and the intestinal contractility was measured，respectively at 1st（C group）, 3rd（D group）,7th（E group）. Meanwhile, the contractility of intestinal tissue was measured, the degree of intestinal mucosal membrane injury was evaluated and the expression of c-kit was observed. Results The comparison of normal and model groups in every data’s measurement were significant on statistics (P<0.05),but the comparison of intestinal mucosal membrane injury between E and A group had no significant difference (P>0.05). The contractility of intestinal tissue of E group was higher than D group (P<0.05), and the degree of intestinal mucosal membrane injury was less serious than D group (P<0.01). Besides, the expression of c-kit in E group wass significantly higher than D group (P<0.01). Conclusion Incomplete intestinal obstruction with down-regulated c-kit could be established by the silk method.
Interstitial cells of Cajal / Silk method / c-kit / Guinea pig / Incomplete small intestinal obstruction
[1] Wu CC, Lin YM, Gao JH, et al. Are interstitial cells of Cajal involved in mechanical stress-induced gene expression and impairment of smooth muscle contractility in bowel obstrucion[J]. 2013, PLoS ONE, 8(9):e76222.
[2] Wu B, Liu L, Gao H, et al. Distribution of interstitial cells of Cajal in Meriones unguiculatus and alterations in the development of incomplete intestinal obstruction[J]. Histol Histopathol, 2013, 28(12):1567-1575.
[3] 朱晓艳,李昆, 彭梅芳, 等. 电针足三里穴对不全肠梗阻大鼠小肠Cajal间质细胞影响的免疫组化观察[J]. 中国普外基础与临床杂志, 2012, 19(9): 984-987.
[4] El-Awady SI, El-Nagar M, El-Dakar M, et al. Bacterial translocation in an experimental intestinal obstruction model. C-reaclive protein reliability [J] ? Acta Cirurgica Brasileira, 2009, 24(2):98-106.
[5] Wu CC, Lu YZ, Wu LL, et al. Role of myosin light chain kinase in intestinal epithelial barrier defects in a rat model of bowel obstruction[J]. BMC Gastroenterology, 2010, 10:39-51.
[6] 赵静波, Hans Gregersen. 不同疾病动物模型中的胃肠生物力学和组织结构重构[J]. 医学综述, 2008, 14(23): 3553-3557.
[7] Chui CJ, Mcardle AH, Browm R, et al. Intestinal mucosal lesion in low-flow states [J]. Arch Surg,1970,101:478-483.
[8] Yu XY, Zou CL, Zhou ZL, et al. Phasic study of intestinal homeostasis disruption in experiment intestinal obstruction[J].World J Gastroenterol, 2014, 20(25):8130-8138.
[9] 张林. SCL转基因联合SCF促Cajal间质细胞恢复的在体实验研究[D].重庆:第三军医大学, 2007.
[10]Caroline CA, Hennig GW, Kurahashi M, et al. Relationship between interstitial cells of Cajal, fibroblast-like cells and inhibitory motor nerves in the internal anal sphincter[J].Cell Tissue Res, 2011, 344(1):17-30.
[11]Kondo J, Powell AE, Wang Y, et al. LRIG1 regulates ontogeny of smooth muscle-derived subsets of interstitial cells of Cajal in mice[J]. Gastroenterology, 2015, 149(2):407-419.
[12]Zhu MH, Sung TS, O’Driscoll K, et al. Intracellular Ca2+ release from endoplasmic reticulum regulates slow wave currents and pacemaker activity of interstitial cells of Cajal[J]. AM J Physiol Cell Physiol, 2015, 308(8): C608-620.
[13]Sanders KM, Ward SM. Interstitial cells of Cajal: a new perspective on smooth muscle function[J]. J Physiol, 2006, 576(3):721-726.
[14]Torihashi S, Nishi K, Tokutomi Y, et al. Blockade of kit signaling induces transdifferentiation of interstitial cells of Cajal to a smooth muscle phenotype[J]. Gastroenterol, 1999, 117(1):140-148.
[15]Peng MF, Li K, Wang C, et al. Therapeutic effect and mechanism of electroacupuncture at Zusanli on plasticity of interstitial cells of Cajal: a study of rat ilum[J]. BMC Complement Altern Med, 2014,14:186-196.
[16]Gim H, Nam JH, Lee S, et al. Quercetin inhibits pacemaker potentials via nitric oxide/cGMP-dependent activation and TRPM7/ANO1 channels in cultured interstitial cells of Cajal from mouse small intestine[J]. Cell Physiol Biochem, 2015, 35(6):2422-2436.
[17]Gao J, Sathar S, O’Grady G, et al. Developmental changes in postnatal murine intestinal interstitial cell of Cajal network structure and function[J]. Ann Biomed Eng, 2014, 42(8):1729-1739.
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