Experimental research on the expression of iNOS in the hippocampus of rats after pure cerebral concussion
LI Heng-xi, WU De-ye, LAI Jiang-hua, KANG Li, WUhai-ying, YU Jian-yun, LI Ping
Chinese Journal of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (4) : 402-406.
Experimental research on the expression of iNOS in the hippocampus of rats after pure cerebral concussion
Objective To investigate the expression change pattern and its pathological link between iNOS and PCC though observing expression changes of iNOS (inducible nitric oxide synthase, iNOS) between hippocampus CA1-4 region and upper and lower dentate gyrus regions in the pure cerebral concussion (PCC) model rat. Method PCC model was established insober rats by using metal pendulum closed brain injury blow device, andthen the rats were randomly divided into 3h, 12h, 1d, 2d, 3d, 7d six injury groups (n=5) and a normal control group (n=5). Location and quantitation of iNOS in hippocampal CA1-4 and superior and inferior dentate gyrus between the PCC groups and the normal group were detected immunohistochemical staining and Western Blot. Result Under normal physiological conditions, iNOS positive cells in hippocampus were few, poorly defined and the protein expression was weak. However, 3h after injury, iNOS and the positive protein expression levels in hippocampus increased and reached the peak on 3d and 2d, respectively. There was a significant difference with the normal group (P<0.05), after 2d, the expression of protein was decreased and the positive expression was also decreased after 3 d, but the expression was still higher than that of normal group until 7 d. Conclusion Expression of iNOS in the hippocampus increased at the early stage of PCC damage, suggesting that the inflammatory factor plays an important role in the pathological changes of PCC.
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