Effects of Platelet-rich Fibrin on mandibular distraction osteogenesis and its RANKL expression
FU Ying, DONG Qing-wen, WANG Zhi-ying
Chinese Journal of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (3) : 322-325.
Effects of Platelet-rich Fibrin on mandibular distraction osteogenesis and its RANKL expression
Objective To investigate the effects of PRF on mandibular distraction osteogenesis and the expression of RANKL in the period of distraction osteogenesis, in order to provide a reference for its clinical research and application. Methods unilateral mandibular osteotomies were performed in 20 mature rabbits, the mandibles of 20 rabbits were lengthened by 4 mm using a distractor , PRF were implanted in the osteotomy side of mandibles. Another side of the mature rabbit was cut and a distractor was implanted in the mandible as a control group. The animals were killed on 1, 7, 14, 21 and 8 days of consolidation period. The distracted calluses were harvested and processed for histological and immunohistochemistric study of RANKL. Results The regenerated bone was found in the distraction gap after mandibular lengthening, and RANKL were co-localized in bone marrow lining cells, osteoblasts and newly embedded osteocytes. The RANKL expression increased on 1 and 14 days. At the consolidation period of 1 and 14 days there was significant difference between the experiment group and control group(P<0.05). The comparison of positive cells rate and positive area percentage in distracted calluses on the 28th day after the distraction were performed, and the results showed weak RANKL staining in the distracted callus. Conclusion PRF could accelerate bone formation of mandibular distraction osteogenesis in rabbit. RANKL may play important roles at the early stage of mandibular distraction.
Mandibular osteo-distraction / Platelet-rich fibrin / Receptor activator for NF-KB ligand
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