Developmental expression of calcium activated chloride ion channels Anoctamin5 in mouse skeletal muscle
SONG Hai-yan, ZHANG Yi-min, ZHOU Li, TIAN Yue-min, FU Sheng-qi
Chinese Journal of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (3) : 318-321.
Developmental expression of calcium activated chloride ion channels Anoctamin5 in mouse skeletal muscle
Objective To study the possible role of the calcium dependent Anoctamin5 chloride ion channels in the process of skeletal muscle development. Methods RT-PCR was used to detect Anoctamin5 mRNA expression in skeletal muscle of different development periods in mice, and immunofluorescence and Western blot methods was used to detect Anoctamin 5 protein expression in different development periods of mice skeletal muscle. Results Anoctamin5 mRNA and protein expression in skeletal muscle in mice from 1 day to 6 months, but with the growth of the age, Anoctamin5 mRNA and protein expression underwent a trend of gradual reduction. Conclusion Anoctamin5 plays an important role in the development process of skeletal muscle in mice, which may participate in differentiation, proliferation, injury and repair of skeletal muscle. Our foundings are also with consistent with the patient developed symptoms later caused by Anoctamin5 mutations.
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