The effect of vitamin C on the neurite outgrowth of dorsal root ganglionic neurons
LI Yuan-yuan, WEN Jin-kun, PAN Meng-jie, LI Li-xia,WANG Xiang-hai, QIAN Chang-hui, GUO Jia-song
Chinese Journal of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (3) : 308-311.
The effect of vitamin C on the neurite outgrowth of dorsal root ganglionic neurons
Objective To study the effect of Vitamin C on the neurite outgrowth of dorsal root ganglionic neurons. Methods Neurons harvested from the dorsal root ganglion of newborn SD rat pups were cultured in a basic medium supplemented with a series of concentration of Vitamin C (0, 100, 200, 400μmol/L). 24 hours later, the cultures were immunostained with antibodies of βIII tubulin and RhoA. The βIII tubulin immunostained cultures were double stained with phalloidin. Then, the number and length of the neurites, number of pseudopodium in the growth cone of the cultured neurons, as well as the immunofluorence intensity of RhoA in the neuronal bodies were quantified. Results The number and length of the neurites, number of pseudopodium in the growth cone of the cultured neurons were all increased in Vit. C treated groups compared with the control group. Howerver， the RhoA level was decreased in the Vit. C treated groups. Conclusion Vit.C could promote the neurite outgrowth of cultured dorsal root ganglionic neurons which may result in reducing the expression of RhoA.
Vitamin C / Neuron / Neuriteougrowth / RhoA
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