RNA interference with CRMP5 inhibites outgrowth of neurite in rat hippocampal neurons
LI Su-mei, LI Bin, ZHANG Ji-feng, TAN Ming-hui, WU Feng-ming, GUO Guo-qing
Chinese Journal of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (3) : 303-307.
RNA interference with CRMP5 inhibites outgrowth of neurite in rat hippocampal neurons
Objective To investigate positive effect of CRMP5 gene on neurite outgrowth in rat hippocampal neurons. Methods After selecting an effective siRNA that interfered CRMP5 gene, and fluorescence radix FAM was constructed upon 5′ terminal of the siRNA. The siRNA was then transiently transfected into hippocampal neurons with lipofection reagent. The formation of neurites and branches of hippocampal neurons were observed by confocal microscopy. Results After the siRNAs labeled by FAM were transfected into hippocampal neurons,the FAM was distributed in the neuron cell body and neurite; Then,CRMP5 siRNA confirmed by immunofluorescence could effectively silence CRMP5 protein expression. The neurons had shorter neurites and branches than the control group after silencing CRMP5 gene expression. Compared with the control group, the length of neurites became significantly shorter after the CRMP5 siRNA was transfected into hippocampal neurons (P<0.05). No significant difference was found in the number of primary neurites, while the number of secondary and above neurites had significant difference (P<0.05). Conclusions Silencing of CRMP5 gene was shown to inhibit neurite growth and branch formation of hippocampal neurons.
CRMP5 / RNA interference / Neurite / Neurons / Hippocampus
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