The role of BDNF on demyelination after compressed spinal cord injury in rat
YU Hai-jun，SUN Shan-quan
Chinese Journal of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (2) : 186-190.
The role of BDNF on demyelination after compressed spinal cord injury in rat
Objective The objective of this study was to observe the relationship between the expression of brain-derived neurotrophic factor (BDNF) and the demyelinating of the myelinated nerve fibers after compressed spinal cord injury (CSCI) and to clarify the role of BDNF in demyelination. Methods 60 male SD rats were divided into 3 groups evenly and randomly：a model group，a sham group and a normal group. The CSCI rat model was established by a self-designed compression device. For the model group, 24h spinal cord compression was given; spinal cord exposition was made to the sham group, with no compression; and nothing was done to the normal group. Osmic acid and luxol fast blue (LFB) staining were used for observing the changes of myelinated nerve fibers. Expression of BNDF and MBP(myelin basic protein) were identified by Western-blot analysis. Results 24h after CSCI，the myelinated nerve fibers had no obvious swelling or change in number in the compressed anterior (T11); but there were excessive swelling and decrease in number in the compressed part (T12) and inferior to the compressed part (L1) (P<0.05). In the compressed anterior (T11), MBP expression was unchanged (P>0.05) 24h after CSCI and the expression of BDNF increased (P<0.05). On the contrary, both MBP and BDNF expression decreased in the compressed part(T12) and the compressed posterior(L1) (P<0.05). And the demyelination was more serious in the compressed part with significant reduction in BDNF (P<0.01). Conclusion Decreased expression of BNDF can lead to demyelination after CSCI , while increased expression of BNDF may have protective effect on demyelination.
Compressed spinal cord injury / Demyelination / Brain-derived neurotrophic factor / Myelin basic protein / Rats
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