Influence of neural cadherin protein expression on myocardial fibrosis in rats
Chinese Journal of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (1) : 78-82.
Influence of neural cadherin protein expression on myocardial fibrosis in rats
Objective To investigate the expression of neural cadherin (N-cadherin) on isoproterenol induced myocardial fibrosis in rat, and provide morphological data for the investigation of the cardiac hypertrophy signal transduction mechanism and reversal of cardiac hypertrophy．Methods The rat models of myocardial hypertrophy were prepared by subcutaneous injection with isoprenaline (ISO) to healthy adult SD rats. The pathological changes of the myocardial collagen fiber were observed conventional paraffin section and Masson staining. The expression and distribution of N-cadherin was detected by immunohistoehemistry and immunofluorescence staining．Reverse transcription polymerase chain reaction was used to determine mRNA expression levels of N-cadherin in the myocardial tissues. Image analysis software was used to conduct quantitative analysis. Results Compared with the control group, the positive expression of N-cadherin protein in the experimental group had no significant difference. Furthermore, mRNA expression of N-cadherin in the myocardial tissues of rats was consistent with the immunohistoehemistry results. Conclusion The N-cadherin protein may be necessary for maintaining the structure and physiological function of myocardium.
Neural cadherin / Immunohistohemistry / Immunofluorescence staining / Myocardial fibrosis
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