The protective effect of bone marrow-derived mesenchymal stem cells on acute lung injury after hip fracture in elderly rat
Chinese Journal of Clinical Anatomy ›› 2015, Vol. 33 ›› Issue (6) : 672-676.
The protective effect of bone marrow-derived mesenchymal stem cells on acute lung injury after hip fracture in elderly rat
Objective To investigate the protective effect of bone marrow-derived mesenchymal stem cells on acute lung injury (ALI) after unilateral hip fracture in elderly rat. Methods 15 month old WISTAR rat were randomly divided into three groups: a control group (n=15)、a model group(hip fracture+NS, n=15) and a treated group (hip fracture+BMSCs, n=15). 1 ml BMSCs（2.5×106/ml）were injected through the caudal vein in the treated group, and the same volume of NS was injected into the model group Histologic changes were examined at 24 hours after fracture. And the concentration of MPO and related inflammatory factors in the impaired lung tissue and blood were examined through enzyme-linked immunosorbent assay (ELISA). Results Compared with the control group,the histopathological damage of lung, the concentration of MPO and related inflammatory factors were obviously enhanced（P<0.01）. Compared with the model group,application of BMSCs significantly ameliorated the inflammatory lesions of the lung, increased the concentration of IL-10, and further down-regulated the concentration of MPO and other inflammatory factors（P<0.01）. Conclusions ALI can be induced by hip fracture in aged rats. BMSCs shows a certain protective effect on ALI with down-regulation of inflammatory response in systemic and pulmonary as a possible mechanism.
Aged rats / Hip fracture / Bone marrow-derived mesenchymal stem cells / Acute lung injury
[1] Cummings SR, Rubin SM, Black D. The future of hip fractures in the united states. Numbers, costs, and potential effects of postmenopausal estrogen [J]. Clin Orthop Relat Res, 1990, (252):163-166.
[2] Murphy DK, Randell T, Brennan KL, et al. Treatment and displacement affect the reoperation rate for femoral neck fracture [J]. Clin Orthop Relat Res, 2013,471(8):2691-2702.
[3] Lawrence VA, Hilsenbeck SG, Noveck H, et al. Medical complications and outcomes after hip fracture repair [J]. Arch Intern Ned, 2002,162(18):2053-2057.
[4] 李绍光. 老年髋部骨折后过高死亡风险分析与应用cox-2抑制剂抗炎治疗改善预后的临床观察[D]. 中国人民解放军医学院, 2013.
[5] Foss NB, Palm H, Krasheninnikoff M, et al. Impact of surgical complications on length of stay after hip fracture surgery [J]. Injury, 2007,38(7):780-784.
[6] Di Nicola M, Carlo-Stella C, Magni M, et al. Human bone marrow stromal cells suppress t-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli [J]. Blood, 2002, 99(10):3838-3843.
[7] Corcione A, Benvenuto F, Ferretti E, et al. Human mesenchymal stem cells modulate b-cell functions [J]. Blood, 2006,107(1):367-372.
[8] Perl M, Gebhard F, Knoferl MW, et al. The pattern of preformed cytokines in tissues frequently affected by blunt trauma [J]. Shock, 2003,19(4):299-304.
[9] Tyburski JG, Dente C, Wilson RF, et al. Differences in arterial and mixed venous il-6 levels: The lungs as a source of cytokine storm in sepsis [J]. Surgery, 2001, 130(4):748-752.
[10] Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis [J]. Science, 1997, 275(5302): 964-967.
[11]Brazelton TR, Rossi FM, Keshet GI, et al. From marrow to brain: Expression of neuronal phenotypes in adult mice [J]. Science, 2000, 290(5497):1775-1779.
[12]Krause DS, Theise ND, Collector MI, et al. Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell [J]. Cell, 2001,105(3):369-377.
[13]Nasef A, Mazurier C, Bouchet S, et al. Leukemia inhibitory factor: Role in human mesenchymal stem cells mediated immunosuppression [J]. Cell Immunol, 2008, 253(1-2):16-22.
[14]Rafei M, Hsieh J, Fortier S, et al. Mesenchymal stromal cell-derived ccl2 suppresses plasma cell immunoglobulin production via stat3 inactivation and pax5 induction [J]. Blood, 2008, 112(13):4991-4998.
[15]Lepelletier Y, Lecourt S, Renand A, et al. Galectin-1 and semaphorin-3a are two soluble factors conferring t-cell immunosuppression to bone marrow mesenchymal stem cell [J]. Stem Cells Dev, 2010,19(7):1075-1079.
[16]Nemeth K, Leelahavanichkul A, Yuen PS, et al. Bone marrow stromal cells attenuate sepsis via prostaglandin e(2)-dependent reprogramming of host macrophages to increase their interleukin-10 production [J]. Nat Med, 2009,15(1):42-49.
[17]Ouyang W, Rutz S, Crellin NK, et al. Regulation and functions of the il-10 family of cytokines in inflammation and disease [J]. Ann Rev Immunol, 2011,29: 71-109.
/
| 〈 |
|
〉 |
