LFPEMFs effects on the proliferation and chondrocyte-like differentiation of BMSCs labeled with SPIO
Chinese Journal of Clinical Anatomy ›› 2015, Vol. 33 ›› Issue (6) : 662-666.
LFPEMFs effects on the proliferation and chondrocyte-like differentiation of BMSCs labeled with SPIO
Objective To explore the effects of low frequency pulsed electromagnetic fields（LFPEMFs）on the proliferation and chondrocyte-like cells differentiation of BMSCs labeled with superpar-amagnetic iron oxide nanoparticle（SPIO）. Methods Labeled BMSCs were developed and divided into four groups, followed by different treatments：LFPEMFs group, chondrocyte-induced group, LFPEMFs and chondrocyte-induced group, and control group. The proliferation and chondrocyte-like differentiation of cells were measured accordingly, adopting CCK8, PCR or immunofluorecence staining, respectively. Results Compared with control group, the proliferation of BMSCs increased significantly in LFPEMFs stimulated cells. The expression level of Aggrecan and Collagen Ⅱ mRNA and protein in groups with LFPEMFs stimulation was significantly higher (P<0.05). Conclusions This experiment proves that LFPEMFs can promote the proliferation and chondrocyte-like cells differentiation of SPIO labeled BMSCs.
LFPEMFs / BMSCs / Proliferation / Aggrecan / CollagenⅡ
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