Researching microvascular casting model of oral and maxillofacial regions of Wistar rats
Chinese Journal of Clinical Anatomy ›› 2015, Vol. 33 ›› Issue (5) : 604-607.
Researching microvascular casting model of oral and maxillofacial regions of Wistar rats
Objectives To build the capillaries casting method of Wistar rats maxillofacial microvascular specimens by perfusion combined with scanning electron microscopy (SEM), so that the three-dimensional digital models of capillaries were established. Methods Maxillofacial microvascular specimens of 8 weeks of Wistar rats were resected from the abdominal cavity. The catheter was inserted from ventricle to the aorta. The resin material was filled, and specimen was placed in constant temperature water bath for 24 hours. The sample was divided into some parts, such as skin, tongue, jaw bones. The samples were corroded, washed, dried, and plated gold. The anatomical morphology of vascular casting was observed under scanning electron microscopy, course and adjacent structures. Results Capillary casting specimens of skin, tongue, mandible and maxilla of Wistar rats were successfully prepared. They clearly showed the morphology and course of the capillaries in the specimens. Under the scanning electron microscopy, the micro capillary structures of the capillaries were showed in three dimensions. Conclusion The facial capillaries casting methods in Wistar rats can serve as the basis for 3D digital model of the microvascular morphology and 3D printing of the capillaries.
Microcirculation / Wistar rat / Microvascular casting / Model
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