Role of iNOS during wound healing of skeletal muscle
Chinese Journal of Clinical Anatomy ›› 2015, Vol. 33 ›› Issue (5) : 545-548.
Role of iNOS during wound healing of skeletal muscle
Objective To observe the morphological changes and iNOS expression at cellular damaged zone during the wound healing of denervated muscle; To determine the affection of the expression of iNOS induced by supernatant of macrophages. Methods The damaged denervated muscle, three days after the damaged muscle model of rat was established, was harvested for H.E and immunohistochemical staining of iNOS. The cultured C2C12 cells were treated with supernatant of macrophages. The treated C2C12 cells were analyzed using RT-PCR method. Results New capillaries near the damaged muscle and high expression of iNOS could be observed. The expression of iNOS was different between the damaged and undamaged zones in muscle cells. The supernatant of macrophages could induce the expression of iNOS and MyoD genes. Conclusion The high expression of iNOS can accelerate the regeneration of denervated muscle during wound healing.
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