The effect of Sulindac on proliferation and β-catenin protein expression of human liver cancer cell HepG2
Chinese Journal of Clinical Anatomy ›› 2015, Vol. 33 ›› Issue (4) : 447-450.
The effect of Sulindac on proliferation and β-catenin protein expression of human liver cancer cell HepG2
Objective To observe the effect of Sulindac on proliferation, apoptosis of human liver cancer cell HepG2 and the expression of β-catenin protein, and to investigate the possible anti-cancer mechanism of Sulindac. Methods HepG2 cells were treated with different dose of Sulindac. The inhibitory effect of Sulindac on proliferation was detected by MTT assay. Hoechst 33258 staining was applied to analyze the effect of Sulindac on apoptosis of HepG2 cell. The expression ofβ-catenin protein in the Wnt pathway was detected by RT-PCR and Western blotting. Results Sulindac could inhibit the proliferation of liver cancer cell HepG2 with time and dose dependence. The number of apoptotic cells after treated with Sulindac for 24h was larger than those treated with DMSO. The expression of β-catenin appeared to decrease with the increase of drug concentration. Conclusion Sulindac can inhibit the growth of liver cancer HepG2 cells. It can block the Wnt signal transduction pathway, and reduce the expression ofβ-catenin, which may be the mechanism of its anti-tumor effect.
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