Building the acute lung injury mouse model by LPS direct and indirect challenge
Chinese Journal of Clinical Anatomy ›› 2015, Vol. 33 ›› Issue (4) : 439-443.
Building the acute lung injury mouse model by LPS direct and indirect challenge
Objective Acute lung injury(ALI) that results from acute inflammation of respiratory system is a popular acute and serious pulmonary symptom in clinic. The successful establishment of the mouse model for ALI is essentially important. Methods In this study, we compared the differences between injection LPS from intratrecher (i.t.) and intraperitoneal (i.p.). By histological and pathological evaluation, cytologic reaction of inflammation cells. Change of protein secreted by inflammation cells were adopted to rate the effectiveness of model establishment induced by i.t. and i.p. injection. Results We got more obvious inflammation reaction and more efficient ALI by i.t. injection. On cytologics item, we got more exudation of neutrophil cells in BALF (bronchial alveoli lavage fluid) by i.t. injection. On cytokines item, secreted proteins were obviously increased in bronchoalveoli lavage fluid by i.t. injection. Conclusions Injection LPS by i.t. is a better method for establishment of ALI model, which leads to acute lung injury directly and immediately.
LPS / Acute Lung Injury(ALI) / Intratrecher(i.t.) / Intraperitoneal (i.p.)
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