The improved primary cell culture method of craniopharyngioma
Chinese Journal of Clinical Anatomy ›› 2015, Vol. 33 ›› Issue (3) : 331-335.
The improved primary cell culture method of craniopharyngioma
Objective Using new method to culture the craniopharyngioma cells in order to provide the foundation of clinical and basic researches on cell level. Methods Using new method to culture, purify and pass two pathological patterns of craniopharyngioma cells. The cells were identified by morphology under microscope, immunohistochemistry and proliferation assay. Results The craniopharyngioma cells cultured by the new method were seen around the tumor piece with better state and greater numbers than the cells cultured by the traditional method. These two kinds of cells were determined to be the craniopharyngioma cells. The proliferation assay showed that the cells were still proliferating and dividing in vitro. Conclusions Using new method which was simple and cheap to culture the craniopharyngioma cells could harvest better cells in state and greater in numbers. Though this method could not solve all the problems about cell culture of craniopharyngioma, it could still provide the foundation of clinical and basic researches about tumor biological characteristics on the cell level by establishment of a stable cell line.
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