Tea polyphenol alleviates the chronic alcoholism induced sperm damage
SHANG Yin-Juan, WANG Jian-Jun, WANG Xiang-Hai, WEN Jin-Kun, BO Meng-Jie, JIAN Chang-Hui, KANG Wei-Xiang, CHEN Zhuo
Chinese Journal of Clinical Anatomy ›› 2014, Vol. 32 ›› Issue (5) : 604-608.
Tea polyphenol alleviates the chronic alcoholism induced sperm damage
Objective To explore the preventive role of tea polyphenol (TP) on the chronic alcoholism induced sperm damage and the potential mechanisms. Methods Forty male Kunming mice were randomly divided into chronic alcoholism (CA) group, TP low-dosage (TP-L) group, TP high-dosage (TP-H) group and normal control (NC) group. Eight hours after daily alcohol administration, the mice in CA, TP-L and TP-H groups were treated with water, 1% TP or 2% TP respectively. The mice in NC group were administered with water twice a day. 90 days later, sperm suspension was prepared to detect sperm density, activity ratio and malformation ratio; Bcl-2 and Bax expression were observed in testicular tissue via immunohistochemistry. Results In CA group, the density, activity ratio of sperm and ratio of Bcl-2 positive cells in testicular tissue were lower, the ratios of sperm malformation and Bax positive cells in testicular tissue were higher than that of NC group. Above changes were reversed in the TP-L and TP-H groups. Moreover, this benign effect showed a positive correlation with dosage. Conclusion Tea ployphenol could alleviate alcoholism induced sperm damage. This effect might relate with the Bcl-2 and Bax expression during spermatogenesis.
Tea polyphenols / Alcoholism / Sperm / Testis / Bcl-2 / Bax
[1] Kurus M, Ugras M, Ates B, et al. Apricot ameliorates alcohol induced testicular damage in rat model
[J].Food Chem Toxicol, 2009,47(10):2666-2672.
[2] Oliva SU, Messias AG, Silva D, et al. Impairment of adult male reproductive function exposed to ethanol since puberty
[J]. Reprod Toxico, 2006, 22(4):599-605.
[3] Lee HY, Naseer MI, Lee SY, et al. Time-dependent effect of ethanol on GnRH and GnRH receptor mRNA expression in hypothalamus and testis of adult and pubertal rats
[J]. Neurosci Lett, 2010,471(1): 25-29.
[4] Giannessi F, Giambelluca MA, Grasso L, et al. Curcumin protects Leydig cells of mice from damage induced by chronic alcohol administration
[J]. Med Sci Monit, 2008,14(11):BR237-242.
[5] Mukhtar H, Ahmad N. Tea polyphenols: prevention of cancer and optimizing health
[J]. Am J Clin Nutr, 2000, 71(6 Suppl):1698S-1702S.
[6] Darvesh AS, Bishayee A. Chemopreventive and therapeutic potential of tea polyphenols in hepatocellular cancer
[J]. Nutr Cancer, 2013, 65(3):329-344.
[7] Hugel HM, Jackson N. Redox chemistry of green tea polyphenols: therapeutic benefits in neurodegenerative diseases
[J]. Mini Rev Med Chem, 2012, 12(5):380-387.
[8] Heinrich U, Moore CE, De Spirt S. et al. Green tea polyphenols provide photoprotection, increase microcirculation, and modulate skin properties of women
[J]. J Nutr, 2011,141(6): 1202-1208.
[9] Shen CL, Yeh JK, Samathanam C, et al. Green tea polyphenols attenuate deterioration of bone microarchitecture in female rats with systemic chronic inflammation
[J]. Osteoporos Int, 2011, 22(1):327-337.
[10] 王文祥, 张文昌, 廖惠珍. 茶多酚对镉致大鼠精子损伤的保护作用
[J].福建医科大学学报, 2011, 45(5): 319-322, 326.
[11] 江莉, 李慕军, 李娜, 等. 密度梯度离心加上游法处理对精子形态学的影响
[J].广西医学, 2012, 34 (1):38-40.
[12] Wallock-Montelius LM, Villanueva JA, Chapin RE, et al. Chronic ethanol perturbs testicular folate metabolism and dietary folate deficiency reduces sex hormone levels in the Yucatan micropig
[J].Biol Reprod, 2007, 76(3):455-465.
[13] Vergara SP, Lizama C, Brouwer-Visser J, et al. Expression of BCL-2 family genes in germ cells undergoing apoptosis during the first wave of spermatogenesis in the rat
[J]. Andrologia, 2011, 43(4):242-247.
[14] Meehan T, Loveland KL, de Kretser D, et al. Developmental regulation of the bcl-2 family during spermatogenesis: insights into the sterility of bcl-w-/- male mice
[J]. Cell Death Differ, 2001, 8(3):225-233.
[15] Yamamoto CM, Hikim AP, Lue Y, et al. Impairment of spermatogenesis in transgenic mice with selective overexpression of Bcl-2 in the somatic cells of the testis
[J]. J Androl, 2001, 22(6):981-991.
[16] Yuan H, Deng Y, Yuan L, et al. Gynostemma pentaphyllum protects mouse male germ cells against apoptosis caused by zearalenone via Bax and Bcl-2 regulation
[J]. Toxicol Mech Methods, 2010, 20(3):153-158.
[17] Albrecht H, Tschopp J, Jongeneel CV. Bcl-2 protects from oxidative damage and apoptotic cell death without interfering with activation of NF-kappa B by TNF
[J]. FEBS Lett, 1994, 351(1):45-48.
/
| 〈 |
|
〉 |
