Study of the effect of Nix and miR-520h on glioma
HU Xiao-Fang, WANG Yong-Kuai, QIU Xiao-Zhong, WANG Guo-Bao
Chinese Journal of Clinical Anatomy ›› 2014, Vol. 32 ›› Issue (5) : 590-594.
Study of the effect of Nix and miR-520h on glioma
Objective To evaluate the biological function of Nix and its relation to microRNA miR-520h in glioma. Methods 46 glioma specimens were selected to detect the expression of Nix by Western blotting and the microRNA expression by traditional RT-PCR．The shRNA were used to knockdown Nix of U251 glioblastomacell line, and the protein level of Nix was detected by Western blotting. In the presense of miR-520h inhibitor．The protein level of Nix and NF-κB was detected to evaluate their interaction in the U25l cell. Results Both RT-PCR analysis and Western blotting showed that high expression of miR-520h was always accompanied with high expression of Nix proteins. Under hypoxic conditions, Western blotting showed that levels of both Nix and p-NF-κB/p65 in Nix-wt U251 glioblastoma cells were significantly higher than those in Nix-kn U251 glioblastoma cells. Furthermore，200 nmol/L of has-miR-520h inhibitor caused low expression of Nix and high expression of p-NF-κB/p65 under hypoxic conditions. Conclusion MiR-520h is related to the expression of Nix protein in the tumorigenesis of glioma. MiR-520h may be a strong tumor activator through promoting Nix biosynthesis .
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