MicroRNA-128 regulates differentiation of bone marrow strowal cells induced by Salvia miltiorrhiza
HUANG Chao, ZHANG Zhi-Jiang, TU Lei, XIE Cai-Jun, CHU Dong-An, BANG Zi-Zhuang, CHEN Cha-Han
Chinese Journal of Clinical Anatomy ›› 2014, Vol. 32 ›› Issue (4) : 427-431.
MicroRNA-128 regulates differentiation of bone marrow strowal cells induced by Salvia miltiorrhiza
Objective To study the regulation of microRNA-128 on differentiation of rat BMSCs into neuron-like cells induced by Salvia miltiorrhiza. Methods Rat BMSCs were isolated, purified and identification in vitro. When the cells reached 80% confluence, microRNA-128 inhibitors were transfected by Lipofectamin 2000. Transfected and untransfected cells were pre-induced for 24h. Then the pre-induced cells induced for 5h by Salvia miltiorrhiza. According to the above experiment, cells were divided into four groups: a control group, an induced group, a transfected group, and an induced-transfected group. To identify the phenotypes, flow cytometry analysis was performed. The expression of microRNA-128 and mRNA for NSE, Nestin and β3-tubulin was detected by qPCR. NSE, Nestin and β3-tubulin protein expression were determined by western blot. Results Most cells showed the characteristic phenotypes of BMSCs positive for CD29 and CD90 (99.17%) and negative for CD11b and CD45 (99.21%). The microRNA-128 expression of the induced group and the transfected group were found to be significantly higher than the control group. The mRNA and protein expression of NSE, Nestin and β3-tubulin were found to be significantly higher in the induced group and in the transfected group (P<0.05). Conclusions Salvia miltiorrhiza can inhibit microRNA-128 expression of BMSCs to increase neural marker protein expression to promote differentiation of BMSCs into neuron-like cells.
Salvia miltiorrhiza / BMSCs / microRNA-128 / Neuron / Cell differentiation
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