Objective To introduce the surgical skill and clinical experience of transperitoneal laparoscopic anatomic retro-adrenalectomy. Methods From January 2009 to January 2013, 47 patients underwent transperitoneal laparoscopic anatomic retro-adrenalectomy. Also, 36 patients underwent posterior laparosciopic adrenalectomy. Clinical data and surgical outcomes were compared retrospectively. Results Patients in the transperitoneal laparoscopic anatomic retro-adrenalectomy group were all successful in surgery. There was significant difference in mean operative time(45.6±23.4 min vs.115.5±18.2 min)and intraoperative blood loss(25.3±10.6 ml vs.110.6±30.3 ml)(P<0.001). No postoperative complications occurred in the transperitoneal laparoscopic anatomic retro-adrenalectomy group(0 vs.13.8%). Conclusions The key advantage to this technique is its minimally invasive approach, shortened surgical time and reduced blood loss. We believe this approach to be a viable and safe option in the removal of benign adrenal lesions.