Bmi -1,hTERT expression in breast cancer cells and its relationship with apoptosis
LIU Chun-Ling, LI Xiao-Chi, YANG Xu, GAO Feng-Lan, DU Hua-Zhen
Chinese Journal of Clinical Anatomy ›› 2014, Vol. 32 ›› Issue (1) : 61-66.
Bmi -1,hTERT expression in breast cancer cells and its relationship with apoptosis
Objective To study the expression changes of proto-oncogene mi-1 and hTERT, and its relationship with apoptosis and to discuss their roles in the occurrence and development of breast cancer. Methods MTT was used to detect the inhibition of breast cancer cell proliferationand the drug concentrations in the experiment; RT-PCR and Western Blot were used to detect the protein and mRNA expression. TUNEL and Flow cytometry were used to detect the apoptosis situation. Results 5-FU could significantly inhibit the growth of breast cancer cell. The differences of OD value between the groups were statistically significant (P<0.05). Under the intervention of 5-Fu, the mRNA and Western Blot expression levels of Bmi-1 and hTERT gradually decreased, and the apoptosis index increased, which exhibited great statistical significance (P<0.05) compared with the control group. Bmi-1 and hTERT showed a positive correlation (P＜0.05),and their expression had a negative correlation with the apoptosis through the statistical analysis. Conclusion The down-regulation of Bmi-1 and hTERT expression in breast cancer cells can promote the apoptosis.
Breast cancer / Bmi-1 / hTERT / Apoptosis
[1] Haupt Y, Bath ML, Harris AW, et al. bmi-1 transgene induces lymphomas and collaborates with myc in tumorigenesis
[J]. Oncogene, 1993, 8(11): 3161-3164.
[2] Alkema MJ, van der Lugt NM, Bobeldijk RC, et al. Transformation of axial skeleton due to overexpression of bmi-1 in transgenic mice
[J]. Nature, 1995, 374(6524): 724-727.
[3] Hosen N, Yamane T, Muijtjens M, et al. Bmi-1-green fluorescent protein-knock-in mice reveal the dynamic regulation of bmi-1 expression in normal and leukemic hematopoietic cells
[J]. Stem Cells, 2007, 25(7): 1635-1644.
[4] Chen YC, Chang CJ, Hsu HS, et al. Inhibition of tumorigenicity and enhancement of radiochemosensitivity in head and neck squamous cell cancer-derived ALDH1-positive cells by knockdown of Bmi-1
[J]. Oral Oncol, 2010, 46(3): 158-165.
[5] Xu W, Zhou H, Qian H, et al. Combination of circulating CXCR4 and Bmi-1 mRNA in plasma: A potential novel tumor marker for gastric cancer
[J]. Mol Med Report, 2009, 2(5): 765-771.
[6] Effendi K, Mori T, Komuta M, et al. Bmi-1 gene is upregulated in early-stage hepatocellular carcinoma and correlates with ATP-binding cassette transporter B1 expression
[J]. Cancer Sci, 2010, 101(3): 666-672.
[7] Choi YJ, Choi YL, Cho EY, et al. Expression of Bmi-1 protein in tumor tissues is associated with favorable prognosis in breast cancer patients
[J]. Breast Cancer Res Treat, 2009, 113(1): 83-93.
[8] Bhattacharyya J, Mihara K, Ohtsubo M, et al. Overexpression of BMI-1 correlates with drug resistance in B-cell lymphoma cells through the stabilization of survivin expression
[J]. Cancer Sci, 2012, 103(1): 34-41.
[9] Feng Y, Song LB, Guo BH, et al.
[Expression and significance of Bmi-1 in breast cancer]
[J]. Ai Zheng, 2007, 26(2): 154-157.
[10]Winnikow EP, Medeiros LR, Edelweiss MI, et al. Accuracy of telomerase in estimating breast cancer risk: a systematic review and meta-analysis
[J]. Breast, 2012, 21(1): 1-7.
[11]Zhang FB, Sui LH, Xin T. Correlation of Bmi-1 expression and telomerase activity in human ovarian cancer
[J]. Br J Biomed Sci, 2008, 65(4): 172-177.
[12]战怀兵, 蔡曦光, 吴凤铭. Bmi-1和hTERT在非小细胞肺癌中的表达及相关性研究
[J]. 临床肿瘤学杂志, 2009, 14(1): 10-14.
[13] Dimri GP, Martinez JL, Jacobs JJ, et al. The Bmi-1 oncogene induces telomerase activity and immortalizes human mammary epithelial cells
[J]. Cancer Res, 2002, 62(16): 4736- 4745.
[14] Haga K, Ohno S, Yugawa T, et al. Efficient immortalization of primary human cells by p16INK4a-specific short hairpin RNA or Bmi-1, combined with introduction of hTERT
[J]. Cancer Sci, 2007, 98(2): 147-154.
[15] Jiang YA, Luo HS, Zhang YY, et al. Telomerase activity and cell apoptosis in colon cancer cell by human telomerase reverse transcriptase gene antisense oligodeoxynucleotide
[J]. World J Gastroen terol,2003, 9(9): 1981-1984.
/
| 〈 |
|
〉 |
