Preparation and in-situ mineralization of sodium hyaluronate and chitosan
LIN Yong-Xin, DING Zhi-Yong, ZHOU Xiao-Bin, SUN Guo-Dong, LI Zhi-Zhong, XIE Bi-Hua, MA Chun-Yan, ZHOU Chang-Ren, LI Li-Hua
Chinese Journal of Clinical Anatomy ›› 2014, Vol. 32 ›› Issue (1) : 52-56.
Preparation and in-situ mineralization of sodium hyaluronate and chitosan
Objective To imitate the structure and composition of natural bone，a ternary composite scaffold was constructed in this paper. Methods Sodium hyaluronate (HA) solution with calcium chloride were added dropwise to chitosan (CS) solution with sodium dihydrogenphosphate solution under high speed stirring. Then the mixture was freeze-dried and then aged in ethanol/water/ urea solution to prepare the HA/CS/HAP porous composite scaffold in situ. The morphology, composition and mechanical property were studied in this research. Results SEM results showed the scaffold was porous, sponge-like with uniform pores with diameter of 100-200 µm. EDS results indicated DCPD was first formed after freeze-drying and the extension of aging time contributed to the transition from DCPD to HA. Conclusion The HA/CS/HAP porous composite scaffold can be used as a basic scaffold material in tissue engineering.
Hydroxyapatite / Chitosan / Hyaluronate / In-situ mineralization / Bone tissue
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