Change of OPN expression and distribution during orthodontic tooth movement with application of implant anchorage in rats
HUANG Min, SHI Jian, DIAO Yue
Chinese Journal of Clinical Anatomy ›› 2014, Vol. 32 ›› Issue (1) : 48-51.
Change of OPN expression and distribution during orthodontic tooth movement with application of implant anchorage in rats
bjective The aim of this study was to investigate the osteopontin (OPN) expression in the periodontal tissues and to understand its possible roles in alveolar bone remodeling through orthodontic tooth movement (OTM) with application of implant anchorage in rats. Methods Orthodontic force with application of implant anchorage was used to move mesially the right mandibular molars of the rats. And the left mandibular was the control. The rats were sacrificed after 2d、4d、7d and 14 d respectively. This procedure was assessed by haematoxylin and eosin was performed to assess the general morphologic change of the mandible and immunohistochemical detection was performed for detection of osteopontin (OPN). Results Osteoblasts increased in number on the tension side of the periodontal ligament space whereas osteoclast increased in number in the compression side. Immunohistochemistry shows that OPN was detectable in the tension region during OTM, but absent in the control group. The expression intensity increased over time and peaked at 7d of OTM. Conclusion The changing tendency of OPN was consistent with the change of bone remodeling and the number of positive osteoblasts. OPN may play an important role in the bone remodeling during orthodontic tooth movement.
Micro-implant anchorage / Orthodontic tooth movement / Osteopontin
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