Distribution and significance of dimer interstitial cells of Cajal in the guinea pig bladder
WANG Jiang-Beng, WANG Qi-Zhang, DING Guo-Fu
Chinese Journal of Clinical Anatomy ›› 2014, Vol. 32 ›› Issue (1) : 45-47.
Distribution and significance of dimer interstitial cells of Cajal in the guinea pig bladder
Objective To observe the distribution of dimer interstitial cells of Cajal (ICC) in the different organizations and parts in guinea pig bladder. Methods The guinea pig bladder sections were made and the distribution of dimer ICC within different organizations were observed using electron microscope; The cryostat tissue sections of guinea pig bladder were made and dimer ICC marked with c-Kit antibody in different bladder parts were observed with immunofluorescence. Results Dimmer ICC, under electron microscope, was mainly distributed in the submucosa of detrusor while monomer ICC was mainly distributed between detrusor bundles. Under high-powered fields of confocal microscopy, the number of dimer ICC in bladder tip was 3.47±0.53 , bladder body was 1.57±0.45 and bladder neck was 0.49±0.19. Dimer ICC density in the bladder tip was significantly higher than that in the bladder body and neck (P <0.01). Conclusion Dimer ICC, mainly located in the submucosa of bladder tip, might accept the tension stimulation from mucous and then trigger the contraction of bladder tip as pacemaker cells.
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