促红细胞生成素导致肥胖小鼠棕色脂肪PPAR γ、UCP1和β3-AR的表达变化

doi:10.13418/j.issn.1001-165x.2017.06.011

中国临床解剖学杂志 ›› 2017, Vol. 35 ›› Issue (6): 645-649.doi: 10.13418/j.issn.1001-165x.2017.06.011

促红细胞生成素导致肥胖小鼠棕色脂肪PPAR γ、UCP1和β3-AR的表达变化

苗莹莹¹，　鲁明¹，　赵俊强²

1.新乡医学院三全学院人体解剖学教研室；　2.新乡医学院三全学院教育技术中心, 河南新乡 453003

收稿日期:2017-07-19 出版日期:2017-11-25 发布日期:2017-12-30
通讯作者: 赵俊强，副教授，E-mail：131050@xxmu.edu.cn
作者简介:苗莹莹，讲师，研究方向：解剖生理学，E-mail：xinyijiepou2007@126.com
基金资助:
2017年河南省软科学研究计划课题（17240041049　5）；国家自然科学基金资助项目（81600677）；河南省医用组织再生重点实验室开放课题（KFKT16003）

Changes of PPAR γ, UCP1 and β3-AR mRNA expression of HFD-induced mice with EPO injection

MIAO Ying-ying¹, LU Ming¹, ZHAO Jun-qiang²

1.Department of Anatomy of Sanquan College, 2.Modern Education Center, Xinxiang Medical University, Xinxiang453003, China

Received:2017-07-19 Online:2017-11-25 Published:2017-12-30

摘要/Abstract

摘要：

目的　观察注射促红细胞生成素（Erythropoietin，EPO）后，高脂喂养（high fat diet, HFD）的肥胖小鼠模型体重、脂肪含量变化以及白色脂肪组织、棕色脂肪组织中过氧化物酶体增殖激活受体γ（peroxisome proliferator-activated receptor γ，PPAR γ）、解偶联蛋白1（uncoupling protein 1，UCP1）、β3肾上腺素能受体（β3 adrenoceptor, β3-AR）mRNA以及蛋白的表达变化，为EPO治疗肥胖的机制提供线索。方法　20只高脂喂养的C57BL/6J雄鼠随机分为对照组（HFD-Con）和EPO组（HFD-EPO）。4周后比较两组动物的体重，实时定量PCR方法检测皮下脂肪、附睾脂肪以及棕色脂肪中的PPAR γ、UCP1和β3-AR mRNA表达变化。结果　4周后，与对照组比较，EPO组体重（24.53±2.03）g，HFD-Con组体重为（31.78±1.68）g，P<0.001。HFD-EPO组动物的皮下脂肪与附睾脂肪重量均显著减少，P<0.001。HE染色结果显示，相较于HFD-Con组，HFD-EPO组动物的脂肪细胞减小。半定量PCR和Western blotting结果显示，皮下脂肪组织与附睾脂肪组织中的PPAR γ、UCP1、β3-AR mRNA以及蛋白的表达均无显著性改变，而棕色脂肪中的PPAR γ、UCP1、β3-AR的mRNA以及蛋白表达均显著增加。结论　棕色脂肪中PPARγ、UCP1、β3-AR表达变化可能与高脂喂养后注射EPO导致体重减轻存在一定的联系。

关键词: , 高脂喂养, 　促红细胞生成素, 　过氧化物酶体激活增殖受体 &gamma, 　解偶联蛋白1, 　&beta, 3肾上腺素能受体

Abstract:

Objective　Erythropoietin (EPO) has been reported to have a beneficial effect on obesity. The aim of the study is to investigate the changes of body weight and fat content of EPO in high fat diet (HFD) mice, the expression of PPAR γ, UCP1, β3-AR mRNA in white adipose tissue (WAT) and brown adipose tissue (BAT). Methods 20 HFD-fed C57BL/6J male mice were randomly divided into control group (HFD-Con) and EPO group (HFD-EPO). The expression of PPAR γ, UCP1 and β3-AR mRNA in subcutaneous fat, epididymal fat and brown fat were detected by real-time quantitative RT-PCR. Results Compared with the control group, the body weight of the EPO mice was (24.53±2.03) g and the control group was (31.78±1.68) g. The weight of subcutaneous fat and epididymal fat in HFD-EPO group were significantly decreased. HE staining showed that the size of adipocytes of HFD-EPO group was decreased compared with HFD-Con group. Quantitative real-time PCR results showed that the expression of PPAR γ, UCP1 and β3-AR mRNA in subcutaneous adipose tissue and epididymal adipose tissue did not change significantly, while the expression of PPAR γ, UCP1 and β3-AR in brown fat were significantly increased. Conclusion　The expression of PPAR γ, UCP1 and β3-AR in brown fat may be related to the weight loss caused by EPO injection after high fat diet.

Key words: High fat diet, 　Erythropoietin, 　Peroxisome proliferator-activated receptors &gamma, 　Uncoupling protein 1, 　&beta, 3-adrenergic receptor

苗莹莹,鲁明,赵俊强. 促红细胞生成素导致肥胖小鼠棕色脂肪PPAR γ、UCP1和β3-AR的表达变化[J]. 中国临床解剖学杂志, 2017, 35(6): 645-649.

MIAO Ying-ying, LU Ming, ZHAO Jun-qiang. Changes of PPAR γ, UCP1 and β3-AR mRNA expression of HFD-induced mice with EPO injection[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(6): 645-649.

参考文献

[1] van Marken Lichtenbelt WD, Vanhommerig JW, Smulders NM, et al. Cold-activated brown adipose tissue in healthy men [J]. N Engl J Med, 2009, 360 (15):1500-1508.
[2] Virtanen KA, Lidell ME, Orava J, et al. Functional brown adipose tissue in healthy adults [J]. N Engl J Med, 2009, 360(15):1518-1525.
[3] Cypess AM, Lehman S, Williams G, et al. Identification and importance of brown adipose tissue in adult humans [J]. N Engl J Med, 2009, 360(15):1509-1517.
[4] Cazzola M. How and when to use erythropoietin [J]. Curr Opin Hematol, 1998, 5(2):103-108.
[5] Katz O, Stuible M, Golishevski N, et al. Erythropoietin treatment leads to reduced blood glucose levels and body mass: insights from murine models [J]. J Endocrinol, 2010, 205(1):87-95.
[6] Teng R, Gavrilova O, Suzuki N, et al. Disrupted erythropoietin signalling promotes obesity and alters hypothalamus proopiomelanocortin production [J]. Nat Commun, 2011, 2:520.
[7] Kotanko P, Thijssen S, Levin NW. Association between erythropoietin responsiveness and body com- position in dialysis patients [J]. Blood Purif, 2008, 26(1):82-89.
[8] Townsend KL, Tseng YH. Of mice and men: novel insights regarding constitutive and recruitable brown adipocytes [J]. Int J Obes Suppl, 2015, 5(Suppl 1):S15-20.
[9] Lee YH, Jung YS, Choi D. Recent advance in brown adipose physiology and its therapeutic potential [J]. Exp Mol Med, 2014, 46:e78.
[10] Saito M. Human brown adipose tissue: regulation and anti-obesity potential [J]. Endocr J, 2014, 61(5):409-416.
[11] Liu Y, Luo B, Shi R, et al. Nonerythropoietic erythropoietin-derived peptide sup-presses adipogenesis, inflammation, obesity and insulin resistance [J]. Sci Rep, 2015, 5:15134.
[12] Alnaeeli M, Raaka BM, Gavrilova O, et al. Erythropoietin signaling: a novel regulator of white adipose tissue inflammation during diet-induced obesity [J]. Diabetes, 2014, 63(7):2415-2531.
[13] Christensen B, Nellemann B, Jorgensen JO, et al. Erythropoietin does not activate erythropoietin receptor signaling or lipolytic pathways in human subcutaneous white adipose tissue in vivo [J]. Lipids Health Dis, 2016, 15(1):160.
[14] Hu E, Kim JB, Sarraf P, et al. Inhibition of adipogenesis through MAP kinase-mediated phosphorylation of PPARgamma [J]. Science, 1996, 274(5295):2100-2103.
[15] Sugimoto S, Nakajima H, Kodo K, et al. Miglitol increases energy expenditure by upregulating uncoupling protein 1 of brown adipose tissue and reduces obesity in dietary-induced obese mice [J]. Nutr Metab (Lond), 2014 , 11(1):14.
[16] Sell H, Deshaies Y, Richard D. The brown adipocyte: update on its metabolic role [J]. Int J Biochem Cell Biol, 2004, 36(11):2098-2104.

促红细胞生成素导致肥胖小鼠棕色脂肪PPAR γ、UCP1和β3-AR的表达变化

Changes of PPAR γ, UCP1 and β3-AR mRNA expression of HFD-induced mice with EPO injection

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	江剑宏, 段仁鹏, 李晓锋. 国人胆道三维重建解剖变异应用研究[J]. 中国临床解剖学杂志, 2024, 42(1): 1-4.
[2]	郭红志, 王瑜, 张加琪, 梁海彬, 马梓玮, 冯伟, 吴忧, 司子燚. 血管铸型结合CT三维模型评估先兆子痫胎盘血管结构[J]. 中国临床解剖学杂志, 2024, 42(1): 5-10.
[3]	李佳伟, 张静, 李灿然, 兰文杰, 籍庆余, 郭志勇, 张云凤, 刘启, 陈清威, 李筱贺. 蒙古族人群股骨近端解剖参数X线测量[J]. 中国临床解剖学杂志, 2024, 42(1): 11-16.
[4]	李琨, 张燕, 郭冉, 郝咪咪, 吴轩宇, 徐艺芳, 王超群, 马文童, 张灵淇, 杨宏宇, 李志军, 张少杰, 王星. 儿童及青少年枕颈角与后枕颈角数字化测量及其临床意义[J]. 中国临床解剖学杂志, 2024, 42(1): 17-20.
[5]	李文, 杨思艺, 黄蕾, 卿霁雯, 蒋松涛, 张磊. 基于MRI探讨Lisfranc韧带的形态学特点及临床意义[J]. 中国临床解剖学杂志, 2024, 42(1): 21-25.
[6]	李凡凡, 徐阳, 王晓旭. 紫草素调节Nrf2/HO-1信号通路对实验性大鼠肉芽肿性小叶性乳腺炎的治疗作用研究[J]. 中国临床解剖学杂志, 2024, 42(1): 26-32.
[7]	王兴航, 丁佳媛, 李放, 包翠芬, 阎丽菁. 人参皂苷Rg1通过抑制NLRP3炎症小体途径减轻氧糖剥夺/复供后小胶质细胞炎症反应[J]. 中国临床解剖学杂志, 2024, 42(1): 33-41.
[8]	程莹莹, 武建军, 蔡海燕, 焦旭文, 马江波, 丁银秀. 体外炎性诱导星形胶质细胞激活及功能分析[J]. 中国临床解剖学杂志, 2024, 42(1): 42-46.
[9]	祁志, 杨力, 贾秋叶, 陈浩伦, 段兆达, 吴春云. 依达拉奉经MAPKs通路对脂多糖激活的小胶质细胞Sirt3表达调控[J]. 中国临床解剖学杂志, 2024, 42(1): 47-53.
[10]	张晶晶, 王洪新. 黄芩苷通过PDGF/P38 MAPK信号通路对肺动脉高压大鼠的保护作用[J]. 中国临床解剖学杂志, 2024, 42(1): 54-58.
[11]	乐钦, 陈荣丽, 熊婧熙, 王婷怡, 蔡志恒, 易秋实, 曾心怡. 白芨多糖对大鼠跨区皮瓣choke区的影响[J]. 中国临床解剖学杂志, 2024, 42(1): 59-64.
[12]	李笑予, 张磊, 付磊, 李东波, 汪国友. 三步接骨法治疗Sanders Ⅱ型跟骨骨折的有限元研究[J]. 中国临床解剖学杂志, 2024, 42(1): 65-70.
[13]	吴研飞, 马剑雄, 卢斌, 王颖, 柏豪豪, 靳洪震, 马信龙. CT后处理技术重建CT值与终板抗压强度的相关性研究[J]. 中国临床解剖学杂志, 2024, 42(1): 71-76.
[14]	庄万强, 唐毅, 骆勇刚, 张辉. 关节镜联合胫骨高位截骨术对髌骨位置及髌股关节功能影响的早中期回顾性研究[J]. 中国临床解剖学杂志, 2024, 42(1): 77-82.
[15]	罗鹰, 窦伟誉, 吴小杭, 陈璟昆, 彭昌贵, 潘剑英 . 微创海马钢板内固定治疗跟骨骨折并发症的影响因素[J]. 中国临床解剖学杂志, 2024, 42(1): 83-88.